Table 1.

Primers Used

Reaction Type	Target	Primers	Sequence (5′–3′)	Annealing Temperature, ºC	Product Size, bp
Real-time PCR	IGF-R1 α	IGF-R1 forward	CAAGCTGTGTGTCTCCGAAA	56	161
		IGF-R1 reverse	TGATTCGGTTCTTCCAGGTC
	GAPDH	GAPDH forward	CATGGCCTTCCGTGTTCCTA	56–63.4	111
		GAPDH reverse	GCGGCACGTCAGATCCA
	TSHR (exons 6–7)	TSHR exon 6 forward	TCCCTGAAAACGCATTCCA	63.4	111
		TSHR exon 6 reverse	GCATCCAGCTTTGTTCCATTG
Genotyping	WT primers 1	TSHR exon 1 forward 1	TGCACCCGGGTCTCTTCCAGC	63.4	241
		TSHR exon 1 reverse 2	AGGCTGGGGATTCGGTGGAGC
	WT primers 2	TSHR exon 1 forward 2	CAGGGCACTGAGAATGTGGCGA	63.4	212
		TSHR exon 1 reverse 2	AGGCTGGGGATTCGGTGGAGC
	TSHR-KO primers	KO forward	AAGTTCATCTGCACCACCG	63.4	170
		KO reverse	TCCTTGAAGAAGATGGTGCG