Table 1.
Major Serum Vitamin D Metabolites in VDR-KO and Transgenic Mouse Models
| Genotype/Diet | n | Age (mo) | 25-OH-D3 (ng/mL) | 24,25-(OH)2D3 (ng/mL) | 25-OH-D3: 24,25-(OH)2D3 (Ratio) | 25-OH-D3-26,23-Lactone (ng/mL) | 25-OH-D3: 25-OH-D3-26,23-Lactone (Ratio) | 1,25(OH)2D3 (pg/mL) |
|---|---|---|---|---|---|---|---|---|
| Wild type + ND | 9 | 2 | 20.2 ± 3.2 | 9.6 ± 2.1 | 2.2 ± 0.2 | 3.0 ± 1.1 | 3.6 ± 1.7 | <300 |
| VDR-KO + ND | 8 | 2 | 8.2 ± 0.9 | 0.3 ± <0.1 | 47.8 ± 8.1 | 0.1 ± <0.1 | 98.0 ± 37 | 3140 ± 470 |
| VDR-KO + RD | 16 | 2 | 10.1 ± 1.8 | 0.1 ± 0.1 | 110.4 ± 30.0 | 0.1 ± <0.1 | 170.1 ± 55.1 | 3420 ± 630 |
| VDR-KO + RDa | 1 | 4 | 10.4 | 0.9 | 11.6 | 2.8 | 3.7 | <300 |
| VDR-KO + RD | 1 | 4 | 9.9 | 0.8 | 12.7 | 2.7 | 3.7 | <300 |
| VDR-KO + RDa | 1 | 4 | 18.1 | 0.2 | 89.4 | <0.1 | 304.3 | 3650 |
| VDR-KO + RD | 1 | 4 | 11.1 | 0.1 | 77.8 | <0.1 | 228.9 | 5160 |
| VDR-KO + RD | 1 | 4 | 15.2 | 0.2 | 87.5 | <0.1 | 314.7 | 2630 |
| VDR-KO + RD | 1 | 6 | 9.9 | 0.7 | 15.2 | 2.2 | 4.5 | <300 |
| VDR-KO + RD | 1 | 6 | 11.6 | 1.1 | 9.6 | 3.2 | 3.6 | <300 |
| VDR-KO + RD | 1 | 6 | 16.0 | 0.2 | 93.9 | <0.1 | 330.1 | 2850 |
| VDR-KO/Tgb mVDR + ND | 7 | 2 | 18.4 ± 4.7 | 8.5 ± 1.8 | 2.1 ± 0.2 | 3.4 ± 0.5 | 5.4 ± 0.8 | <300 |
| VDR-KO/Tg hVDR + ND | 3 | 2 | 22.2 ± 1.2 | 8.9 ± 1.6 | 1.9 ± 0.2 | 2.9 ± 0.3 | 5.9 ± 1.1 | <300 |
| VDR-KO/Tg L233S-hVDR | 5 | 2 | 6.7 ± 0.8 | 0.1 ± <0.1 | 62.3 ± 0.3 | <0.1 | 139.5 ± 16.4 | 3303 ± 656 |
Abbreviations: KO, knockout; ND, normal diet; RD, rescue diet containing high calcium/high lactose.
a
Chromatographic traces for these mice are presented in Figure 3.
b
Transgenic animals (Tg) with mouse(m), human(h), or L233S-modified hVDR (ND).