Table 2.

Average single channel conductance of CP2 toxin in the presence of different NEs in the bathing solution.

NE	Mw	r	G	CP2
				G+NE/G-NE	X (mS/cm)	Filling	%
None			565		103.5
Ethylene glycol	62	0.26	277	0.490	68.8	2.07	99.62
Glycerol	92	0.308	281	0.497	69.6	2.08	100.38
Arabinose	150	0.34	278	0.492	66.7	1.87	90.20
Sorbitol	182	0.39	293	0.519	68.0	1.78	85.78
PEG 200	200	0.43	182	0.322	55.0	2.39	115.08
PEG 400	400	0.7	145	0.257	50.0	2.71	130.59
PEG 600	600	0.78	189	0.334	54.1	2.18	105.18
PEG 1000	1000	0.94	171	0.303	52.3	2.35	113.54
PEG 2000	2000	1.22	437	0.774	53.1	0.31	14.82
PEG 3350	3350	1.44	501	0.887	55.6	0.15	7.14
PEG 6000	6000	2.5	540	0.956	50.5	0.04	2.13

Average single channel conductances (G) were calculated from at least 100 conductance steps. The aqueous phase contained 1 M KCl and the corresponding nonelectrolyte at a concentration of 20% (w/v). Vm = +50 mV; T = 23 ± 1.5°C; Mw, molecular mass; r, hydrodynamic radius; χ is the conductivity of the aqueous solutions. Mw and r, as well as the channel filling (F) and percentage of channel filling (%F) were taken and calculated as described elsewhere (Krasilnikov et al., 1992, 1998; Bárcena-Uribarri et al., 2013).