Figure 3.
Representative curve of oxidation susceptibility of plasma VLDL obtained from LDL receptor knockout (LDLr−/−) and wild type mice. The VLDL oxidation was induced by incubation with CuSO4 (40 μM). Formation of conjugated dienes was followed by absorbance changes at 234 nm along time. The VLDL lag-time to start oxidation was 73 ± 4.8 and 382 ± 79.9 minutes for LDLr−/− and wild type mice, respectively (P = 0.0007). The VLDL oxidation rate, determined by the curve slopes, was about 2-fold greater (P < 0.0001) in the LDLr−/− than wild type VLDL. The n used in this experiment was of 6–8 mice.