Table 1.
Primers sequence for real-time PCR (hAECs).
| Gene | Primer sequence 5′→3′ | Amplicon size (bp) | |
|---|---|---|---|
| Oct4 | Forward | GGCCCGAAAGAGAAAGCGAACC | 224 |
| Reverse | ACCCAGCAGCCTCAAAATCCTCTC | ||
|
| |||
| Nanog | Forward | TTCCTTCCTCCATGGATCTG | 213 |
| Reverse | TCTGCTGGAGGCTGAGGTAT | ||
|
| |||
| SOX2 | Forward | GCCGAGTGGAAACTTTTGTC | 264 |
| Reverse | GTTCATGTGCGCGTAACTGT | ||
|
| |||
| E-cadherin | Forward | TGAGCTTGCGGAAGTCAGTT | 219 |
| Reverse | ACCGTGAACGTGTAGCTCT | ||
|
| |||
| N-cadherin | Forward | CGCCATCCGCTCCACTT | 227 |
| Reverse | GGACTCGCACCAGGAGTAAT | ||
|
| |||
| Vimentin | Forward | CTCTGGCACGTCTTGACCTT | 231 |
| Reverse | ACCATTCTTCTGCCTCCTGC | ||
|
| |||
| CK19 | Forward | CCTACAGCTATCGCCAGTCG | 243 |
| Reverse | TGGTTAGCTTCTCGTTGCCC | ||
|
| |||
| Snail | Forward | CTCGGACCTTCTCCCGAATG | 223 |
| Reverse | TCATCAAAGTCCTGTGGGGC | ||
|
| |||
| Beta-actin | Forward | TCGCCTTTGCCGATCC | 202 |
| Reverse | GAATCCTTCTGACCCATGCC | ||