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Primers sequence for real-time PCR (mice ovaries).
| Gene | Primer sequence 5′→3′ | Amplicon size (bp) | |
|---|---|---|---|
| VEGFA | Forward | GCAGCGACAAGGCAGACTAT | 169 |
| Reverse | AACCTCCTCAAACCGTTGGC | ||
|
| |||
| VEGFR1 | Forward | TCAAGCTAGAGGTGTCCCCG | 152 |
| Reverse | CTCGGCACCTATAGACACCC | ||
|
| |||
| VEGFR2 | Forward | GGCGGTGGTGACAGTATCTT | 152 |
| Reverse | GAGGCGATGAATGGTGATCT | ||
|
| |||
| Beta-actin | Forward | TGGCTCCTAGCACCATGAAG | 193 |
| Reverse | AACGCAGCTCAGTAACAGTCC | ||
