Figure 7. Effects of RA-XII on tumour growth and metastasis in a 4T1-tumour bearing mice model.

Mice were treated with RA-XII at 5, 10 or 20 mg/kg intravenously twice a week for 3 weeks. (A) The levels of serum enzymes, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and creatine kinase (CK) of blood plasma collected from the mice in each group. (B) Body weight and (C) tumour weight of the mice at the end of experiment. Representative photographs of (D) haematoxylin and eosin staining of lungs and livers tissues with arrows showing the tumours, and (E) immunohistochemistry against CD31 in tumour tissues. The histograms summarized the quantified data expressed as mean + SEM of 5 to 7 mice from each group. Statistical differences were determined by One-way ANOVA, followed by Dunnett Test, with *p < 0.05, **p < 0.01, ***p < 0.001 against untreated controls.