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. 2015 Nov 23;5:16940. doi: 10.1038/srep16940

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The levels of IL-1β were analyzed directly in heart homogenate without any additional stimulus and expressed as pg of cytokine per mg of total protein at the 5^th, 10^th, 20^th, and 40^th weeks post-infection. Black bars represent the M₂AChR-immunized groups and white bars represent the pcDNA3-immunized control groups. Bar with a indicates statistically significant differences between WT pcDNA3 and WTM₂AChR groups. The letter d indicates a comparison between the WT M₂AChR and P2×7^−/− M₂AChR groups. N = 5, p < 0.05 was considered statistically significant.