Table 1. Electrocardiogram recordings in Wild Type (WT) and P2×7−/− immunized mice.
| Wild Type | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 wks p.i | 5 wks p.i |
10 wks p.i |
20 wks p.i |
40 wks p.i |
|||||
| pcDNA3 | M2AChR | pcDNA3 | M2AChR | pcDNA3 | M2AChR | pcDNA3 | M2AChR | ||
| RR interval (ms) | 246.47 ± 22.78 | 225.33 ± 14.23 | 260.94 ± 42.24 | 262.98 ± 11.97 | 220.70 ± 13.65 | 175.78 ± 30.50 | 181.16 ± 56.15 | 268.85 ± 38.12 | 250.80 ± 18.90 |
| Heart Rate (BPM) | 248.11 ± 34.94 | 245.33 ± 43.20 | 232.30 ± 35.42 | 222.63 ± 13.17 | 251.96 ± 40.91 | 306.80 ± 65.03 | 342.26 ± 95.11 | 213.77 ± 24.17 | 229.53 ± 43.39 |
| QRS interval (ms) | 10.35 ± 2.26 | 11.22 ± 0.86 | 9.46 ± 1.07 | 10.38 ± 0.95 | 9.07 ± 0.59 | 11.07 ± 0.62 | 10.56 ± 1.09 | 11.03 ± 1.42 | 11.31 ± 1.13 |
| QTc interval (ms) | 44.00 ± 4.29 | 39.39 ± 6.30 | 40.60 ± 5.12 | 44.24 ± 1.85 | 45.23 ± 4.17 | 57.80 ± 12.86 | 56.68 ± 13.24 | 63.66 ± 18.29 | 57.05 ± 5.47 |
| ST Height (mV) | 0.15 ± 0.07 | 0.09 ± 0.07 | 0.11 ± 0.01 | 0.08 ± 0.01 | 0.19 ± 0.04 a | 0.06 ± 0.04 | 0.10 ± 0.02 | 0.13 ± 0.04 | 0.10 ± 0.05 |
| JT interval (ms) | 11.41 ± 2.08 | 11.89 ± 6.25 | 11.12 ± 1.58 | 12.30 ± 1.80 | 12.79 ± 1.49 | 14.79 ± 1.24 | 12.27 ± 3.48 | 18.47 ± 5.50 | 17.44 ± 1.92 |
| P2×7−/− | |||||||||
| RR interval (ms) | 189.63 ± 16.24e | 192.15 ± 16.38 | 208.20 ± 16.36d | 193.43 ± 51.37c | 241.83 ± 25.87b | 152.52 ± 31.71 | 182.00 ± 14.35 | 244.06 ± 17.43 | 239.48 ± 10.43 |
| Heart Rate (BPM) | 311.19 ± 30.14e | 282.78 ± 73.05 | 287.18 ± 20.29 | 353.47 ± 32.13c | 235.90 ± 21.21b | 390.06 ± 85.31 | 303.83 ± 13.45 | 274.57 ± 31.17 | 252.97 ± 5.84 |
| QRS interval (ms) | 8.88 ± 2.03 | 11.64 ± 3.05 | 9.17 ± 2.19 | 9.81 ± 1.37 | 10.73 ± 3.40 | 9.98 ± 1.51 | 10.43 ± 2.07 | 10.05 ± 2.39 | 12.89 ± 2.52 |
| QTc interval (ms) | 48.80 ± 5.05 | 52.58 ± 11.01 | 42.87 ± 4.61 | 61.00 ± 18.85 c | 56.19 ± 6.87 | 63.00 ± 2.79 | 61.40 ± 10.34 | 58.20 ± 8.26 | 57.02 ± 6.16 |
| ST Height (mV) | 0.06 ± 0.06e | 0.00 ± 0.08 | −0.03 ± 0.08d | −0.01 ± 0.04 | −0.02 ± 0.10d | −0.01 ± 0.09 | −0.07 ± 0.03d | −0.01 ± 0.09c | −0.01 ± 0.11 |
| JT interval (ms) | 11.18 ± 3.11 | 11.51 ± 6.98 | 9.43 ± 1.28 | 14.33 ± 6.04 | 15.11 ± 4.32 | 15.34 ± 1.69 | 13.04 ± 3.90 | 18.23 ± 4.26 | 15.00 ± 2.50 |
Comparison between WT pcDNA3 and WT M2AChR and between P2×7−/− pcDNA3 and P2×7−/− M2AChR are marked with the letters a and b, respectively. Comparison between WT pcDNA3 and P2×7−/− pcDNA3 and between WT M2AChR and P2×7−/− M2AChR are marked by the letter c and d, respectively. Comparison between WT and P2×7−/− previously immunization scheme was marked by letter e. p < 0.05 was considered statistically significant difference. N = 10. Wks p.i. – Weeks post-immunization.