Fig. 2.

The kinase inhibitory effects of SB203580 in macrophages. Cells were pretreated with SB203580 for 1 h and stimulated with LPS (50 ng/ml) for 30 min. Cells were then harvested to examine the levels of p38 phosphorylation by western blotting. (A, B) Phosphorylation of p38 in RAW264.7 (A) or mouse resident peritoneal (B) macrophages; ^###p < 0.001 vs. unstimulated controls; **p < 0.01 and ***p < 0.001 vs. cells treated with LPS alone