Fig. 3.

SB203580 differentially modulates LPS-induced pro-inflammatory cytokine production in macrophages. Cells were pretreated with SB203580 at the indicated concentrations for 1 h and then were stimulated by adding LPS (50 ng/ml); culture supernatants were collected 12 h later and concentrations of TNF-α and IL-6 were measured by enzyme-linked immunosorbent assay. Data shown represent (A) TNF-α and (B) IL-6 production by RAW264.7 macrophages, and (C) TNF-α and (D) IL-6 production by mouse resident peritoneal macrophages; ^### p < 0.001 vs. unstimulated controls; *p < 0.05, *p < 0.01, and ***p < 0.001 vs. the LPS alone group