Fig. 4.

SB203580 differentially regulates the transcription of LPS-induced inflammatory cytokine genes in macrophages. Cells were pretreated for 1 h with SB203580 and stimulated for another 4 h with LPS (50 ng/ml). Then, cells were collected to measure the transcript levels of TNF-α and IL-6 by quantitative real-time reverse transcriptase PCR. Data shown represent (A) TNF-α and (B) IL-6 mRNA levels in RAW264.7 macrophages, and (C) TNF-α and (D) IL-6 mRNA levels in mouse resident peritoneal macrophages. For transcript quantification, the mRNA expression data were normalized to the β-actin signal using the 2^-ΔΔCT method; ^### p < 0.001 vs. unstimulated controls; *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the LPS alone group