Table 1.

Primer sequences and optimized annealing temperatures used in the real-time PCR reaction

Gene	Forward sequence	Reverse sequence	Annealing temperature
TLR2	5’-TCCATCCCATGTGCGTGGCC-3’	5’-CAGGACCCCCGTGAGCAGGAT-3’	59°C
NOD1	5’-GCCCCGCGTTCAGGTCGAAA-3’	5’-GTGAGGCGGCTGAAGCAGGG-3’	61°C
TRAF6	5’-AGGGACCCAGCTTTCTTTGTGTGC-3’	5’-CCGTGCGTGGCAGTTCCACC-3’	62°C
HMGB1	5’-GGCAAGTGAGAGCCGGACG-3’	5’-GCTTCTTCTTATGCTCCTCCCGACA-3’	62°C
HSP70	5’-CCATCATCAGCGGACTGTACC-3’	5’-CTGACCCAGACCCTCCCTT-3’	59°C
GAPDH	5’-GTGAAGCAGGCGTCGGAGGG-3’	5’-GCTCTTGCTGGGGCTGGTGG-3’	59°C, 61°C and 62°C