Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 23;5:17062. doi: 10.1038/srep17062

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Challenges to analysis of neuronal structures in images with low SNR. Rows of images: (a–d) Original fluorescent images of neurons analyzed by various methods. (e–h) Edge detection* with manual adjustment of threshold applied to (a–d). (i–l) Skeletonization applied to (a–d) using a low threshold level. (m–p) Skeletonization applied to (a–d) with a high threshold level to remove additional noise. Columns of images: (a,e,i,m) Strong background signal and pixel noise interferes with detection of neuronal structures. Applying a higher threshold to reduce noise (m) results in complete loss of neurite. (b,f,j,n) Inconsistent signal intensity along a neurite leads to a disconnected neurite. (c,g,k,o) False identification of neuronal soma occurs when debris particles are similar in size (indicated by red arrows). (d,h,l,p) Neurites lost or overwhelmed by background noise (indicated by blue arrows). Scale bar = 100 μm. *Edge detection in this study used the Canny operator unless specified otherwise.