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. 2015 Nov 23;5:17062. doi: 10.1038/srep17062

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Copyright © 2015, Macmillan Publishers Limited

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(a) A stack of two-photon laser scanning microscopic images (60 images) shows GFP expressing axons from the mouse neocortical layer 1²². Stacked images are shown in 3D projection and rotated with (b) 0 and (c) 45 degrees. (d) Neurite tracing of (a) using NIA. Analyzed images are shown in 3D projection and rotated with (e) 0 and (f) 45 degrees. For better visualization of traced neurites, we have thickened the detected neurite structures. Further comparison of 3D projections is shown in Videos 1 and 2 (Supporting information). All processes are fully automated.