Figure 4. Effect of 2 weeks of shading on gene expression in Zostera muelleri.

Relative expression of PSI (Photosystem I, reaction center subunit IV, chloroplast precursor, (A) and HSF (Heat Stress Transcription factor A-1d, (B) were normalized to the consensus four most stable reference genes (Poly(A) RNA polymerase, Glyceraldehyde 3-phosphate dehydrogenase, Translation initiation factor 1 subunit beta and Tubulin beta-1 chain). The statistical difference between means is indicated as *P < 0.05. Error bars represent SEM with 3 biological replicates.