Table 1. Reference genes and target genes investigated in Zostera muelleri by using RT-qPCR.
| Name | Accession number | Primer forward sequence | Primer reverse sequence | Length (bp) | Tm | CT | Efficiency (%) |
|---|---|---|---|---|---|---|---|
| S4 | Zoma_Contig219 | ATGGTCTGACA | TGTTATCCAA | 108 | 59.7 | 29 | 114 |
| GAGCGACAA | ACGCATCTCG | ||||||
| AHCY | Zoma_C_c69135 | TTCTTCATCTT | GCATGGAAGGT | 118 | 60.1 | 31 | 113 |
| GCGCATCTG | CTCCAAGTC | ||||||
| 18S | Zoma_C_c67952 | AACGAGACCTC | AAGATTACCCA | 189 | 60.2 | 26 | 88 |
| AGCCTGCTA | AGCCTGTCG | ||||||
| Actin | Zoma_ZMF02257 | TAAGGTCGTTG | ACTCTGCCTTT | 104 | 60.4 | 26 | 110 |
| CTCCTCCTG | GCAATCCAC | ||||||
| PolyA | Zoma_C_c36619 | GCTGCTCGTTC | ATGACCGCCAT | 112 | 59.9 | 29 | 93 |
| AAATTCCTC | TTAATCTGC | ||||||
| GADPH | Zoma_C_c6252 | CGGTTACTGTA | CAAAGGCTGGG | 79 | 59.9 | 25 | 88 |
| GCCCCACTC | ATTGGTTTA | ||||||
| TubB | Zoma_Contig120 | GGACAAATCTT | TCCAGATCCAG | 195 | 60 | 24 | 88 |
| CCGTCCAGA | TTCCACCTC | ||||||
| Calmo | Zoma_B_i07192 | ATCCATCCTGG | CACTGTGATCC | 197 | 60.1 | 23 | 114 |
| TCTTTGTCG | ACTCGTTGG | ||||||
| EloF1 | Zoma_C_c59090 | AAGCAAAGGC | TCTGCTGCCTTC | 82 | 59.9 | 24 | 104 |
| GTCACTTGAT | TTCTCCTC | ||||||
| EloF2 | Zoma_B_i03951 | GGAAGATTTGC | GCAACAAAAC | 174 | 60 | 26 | 102 |
| ACCCAAGAA | CTGCCTTGAT | ||||||
| PSI | Zoma_C_c25681 | GGGAACCAAG | GAATCTCACCA | 121 | 59.7 | 35 | 100 |
| GTGAAGATT | CAACTGGGTA | ||||||
| HSF | Zoma_B_i13463 | GGATGGACCTTCCTAAATCCA | ACACCTTCCGAGTTTTGCAC | 107 | 60.1 | 34 | 95 |
Accession numbers of the closest sequence matches available online in the data repository for Zostera marina EST (http://drzompo.uni-muenster.de/), primers sequences, amplicon length, melting temperature, geometric mean of cycle threshold (CT) and RT-qPCR efficiency are indicated. S4: 30S ribosomal protein S4; AHCY: Adenosylhomocysteinase; 18S: 18S ribosomal protein; PolyA: Poly(A) RNA polymerase; GADPH: Glyceraldehyde 3-phosphate dehydrogenase; TubB: Tubulin beta-1 chain; Calmo: Calmodulin; EloF1b: Translation initiation factor 1 subunit beta; EloF2b: Translation initiation factor 2 subunit beta; PSI: Photosystem I, reaction center subunit IV; HSF: Heat Stress Transcription factor A-1d.