Figure 2.

Fluorescence and absorbance spectra of Rho-MatB. (A) Fluorescence excitation and emission spectra of 1 μM Rho-MatB in 50 mM Hepes at pH 7.0, 100 mM NaCl, 10 mM MgCl₂, and 0.3 mg mL^–1 bovine serum albumin in the absence and the presence of 175 μM ATP. Excitation was at 553 nm for the emission spectra. Emission was measured at 575 nm for the excitation spectra. (B) Absorbance spectra of 1 μM Rho-MatB in the same buffer in the absence and presence of 119 μM ATP.