Table 1. Fluorescence Changes and Dissociation Constants (K_d) for Binding of ATP and Other Nucleotides to Rho-MatB^a.

nucleotide	F+/F–	Kd (μM)
ATP	3.9 ± 0.2	6.4 ± 0.6
ADP	2.6 ± 0.1	428 ± 50
dATP	3.8 ± 0.2	440 ± 53
ATPγS	1.9 ± 0.1	16.2 ± 0.8
AMP-PNP	3.5 ± 0.1	253 ± 11

^aBoth the ratio of fluorescence at the saturating ligand to that in the absence of ligand (F₊/F_–) and the dissociation constants were obtained from fits using a quadratic binding equation to titrations as in Figure 3A. The values are averages of at least three separate measurements.