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. 2015 Nov 23;6:596. doi: 10.3389/fimmu.2015.00596

Figure 1.

Figure 1

Alloantigen-specific RA-iTregs allow the generation of stable mixed chimerism in NM-BMT mice. (A) Schematic representation of NM-BMT regimen. BALB/c mice received NM-BMT regimens that consisted in the administration of low doses of irradiation (3 Gy of TBI at day −1), together with co-stimulation blockade with Abatacept (CTLA4Ig) (24 mg/kg at day 2), and three doses of Rapamycin (5 mg/kg at days −1, 0, and 2). At day 0, mice received 20 × 106 bone-marrow cells from C57BL/6 mice and 2.2 × 106 BALB/c RA-iTreg cells or PBS. (B) Chimerism at 3 weeks post-transplantation was analyzed by the presence of H-2Kb positive cells in blood. (C) Kinetics of mixed chimerism, analyzed as the presence of H-2Kb positive cells in peripheral blood samples from NM-BMT mice treated with (□) or without (●) RA-iTregs. The statistical one-way ANOVA test was used. Bars represent the SE. The data represent seven independent experiments. Rapa, Rapamycin; Aba, Abatacept; RA-iTregs, retinoic acid-induced Tregs cells; BMT: Bone-marrow transplantation. ***p < 0.0001; **p < 0.001; *p < 0.01.