Fig. S3.

p53 knockdown does not rescue stratification defect. (A) Schematic of shRNA-expressing lentiviral constuct to transduce epidermis, with CeFP reporter. (B) Immunofluorescence image of lentivirus-infected Plk4 cOE epidermis with p53 knockdown. (Inset) Regions of low and high infectivity, delineated by the blue dashed line. DAPI is in blue. (C and D) Quantification of apoptotic cells in sagittal sections (C) and spinous layer stratification (D) in embryos transduced with lentivirus expressing either Scramble control or p53 shRNA. Error bars represent SEM.