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. 2015 Nov 4;112(46):E6388–E6396. doi: 10.1073/pnas.1511238112

Fig. 1.

Fig. 1.

ABA-induced gene expression and SnRK2 activity in the WT line and AR7. (A) Results of comparative microarray analysis showing ABA up-regulated and down-regulated transcripts. (B) RNA gel blot analysis of WT and AR7 protonemata. Total RNA isolated from protonemata treated with 10 µM ABA for the indicated times was probed with radiolabeled cDNA fragments of the representative ABA-inducible transcripts, 17B9 (Pp1s294_52V6.1), 6A5 (Pp1s267_21V6.1), LEAIII (Pp1s118_232V6.1), and 19C6 (Pp1s52_261V6.1) (43). (C and D) In-gel kinase assays of WT and AR7 protonema for detection of SnRK2 activity. P. patens protonemata were treated with 10 µM ABA for the indicated times (C) or were subjected to various treatments (D). ABA, 10 µM ABA for 30 min; Cold, 0 °C for 1 h; Desic, desiccation in a laminar flow for 30 min; Man, 0.5 M mannitol for 30 min.