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. 2015 Nov 4;112(46):E6388–E6396. doi: 10.1073/pnas.1511238112

Fig. 5.

Fig. 5.

Phylogenetic and functional analyses of group B3 Raf-like MAPKKKs in land plants. (A) Phylogenetic analysis of plant Raf-like MAPKKKs belonging to groups B1–B4 in P. patens, S. moellendorffii, and A. thaliana. (B and C) Transient assays of P. patens protonemata using various MAPKKK DNA clones. The cDNA clones were fused to the rice actin promoter and were cointroduced with Em-GUS and Ubi-LUC into protonemata. The protonemata were incubated with or without ABA for 1 d and were used for GUS and LUC assays. Error bars indicate the SE (n = 3). ***P < 0.001 and **P < 0.01 compared with the vector control (t-test). (B) Results for ARK and two group B1 MAPKKKs, Pp1s63_65V6.1 and Pp1s75_1V6.1. (C) Results for ARK, two Arabidopsis genes, At1g73660 and At4g24480, and one Selaginella gene, Sm269874.