Fig. S2.

Generation of the ARK knock-in line of P. patens. (A) Schematic representation of the gene knock-in construct of ARK for replacement of Phe (F) at the amino acid position 532 (aa532) with Ser (S). The gene cassette 35Sp-NPT-35Ster was inserted into the position 3′ to the polyadenylation site to select transformants on G418-containing medium. Arrows indicate positions of primers used for PCR analysis. (B) PCR analysis to confirm targeting using the ARK genome-specific primers (primers 1 and 2) and NPT primers (primers 3 and 4).