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. 2015 Nov 4;112(46):E6388–E6396. doi: 10.1073/pnas.1511238112

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Fig. S6. — Phosphorylation of PpSnRK2B analyzed with a nanoLC-MS/MS system. (A) MS/MS spectrum of the phosphopeptide (m/z = 924.46) of MBP-PpSnRK2B generated by GST-ARK^KD phosphorylation. (B) Ion current chromatograms of the phosphopeptide from MBP-PpSnRK2B phosphorylated by GST-ARK^KD (Upper) and total tryptic peptides (Lower). (C) Chromatograms of the peptide derived from autophosphorylated MBP-PpSnRK2B (Upper) and total tryptic peptides (Upper). (D–F) Results of a reference peptide (m/z = 691.87) by the analyses shown in A–C.