Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
AAV capsid engineering through directed evolution. Directed evolution involves (1) random mutagenesis, recombination, and/or DNA shuffling of AAV capsid genes from all AAV serotypes generate a large AAV capsid library with chimeric AAV capsids. (2) This capsid library is applied to (2a) in vitro or (2b) in vivo selection systems, and (3) AAV capsids that successfully overcome the selection criterion are identified.
