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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochem J. 2015 Jul 1;469(1):59–69. doi: 10.1042/BJ20150099

Figure 7. mAb(A)p110γ affects binding of Gβ1γ2 to p87-p110γ.

Figure 7

Purified recombinant p87-p110γ or p101-p110γ (0.375 µg of catalytic p110γ subunit) in the absence or presence of Gβ1γ2 (1.25 µg) and H-Ras (1.25 µg) were subjected to immunoprecipitation (IP) using mAb(A)p110γ as described in the Experimental section. Duplicates of immunoprecipitates were separated by SDS/PAGE (10% acrylamide) followed by immunoblotting (IB) with mAb(B)p110γ. Co-immunoprecipitated Gβ1γ2 and H-Ras were visualized using specific anti-Gβ(1–4) and anti-Ras antibodies. Weak unspecific chemiluminescence signals detected by anti-Ras antibody in PI3Kγ immunoprecipitates in the absence of Gβ1γ2 and H-Ras are caused by light chains of mAb(A)p110γ.