Fig. 8.

Expression of Peste des petits ruminants virus (PPRV) N and H proteins and/or GFP with C77 mAb binding activity in the virus recombinants (positive and negative marker vaccine strains) and parental virus infected cells; VDS cells were infected with viruses at an MOI of 0.01 and fixed 24 h post-infection using 4% paraformaldehyde; cells were stained separately with primary antibodies of mouse anti PPRV H (C77) and mouse anti PPRV N (C11) followed by secondary antibody Alexa Fluor 488 goat anti-mouse (red); cell nuclei were stained with DAPI (blue) and GFP autoflorescence (green); expression pattern of the virus N and H proteins were comparable between the recombinant and parental virus. The anti-PPRV H-mAb could not detect mutated H-protein in rPPRV-C77 infected cells. Autoflorescence of GFP was observed in the rescued rPPRV + GFP (an image with the autoflorescence to show GFP was not taken alongside N antibody staining). Adapted from Muniraju et al. (2014b). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.).