The observed mean survival proportions of Dermanyssus gallinae following the ingestion of blood from hens immunised with recombinant vaccine candidates. Immunisation of hens with the 10 recombinant vaccine candidates and subsequent mite feeding assays were performed in two tranches. Mites were fed with whole heparinised blood from hens immunised with the tranche 1 recombinant vaccine candidates (A: Deg-ASP-1, Deg-GPD-1, Deg-SRP-1, Deg-PUF-1) or tranche 2 (B: Deg-HGP-1, Deg-VIT-1, Deg-PUF-3, Deg-SRP-2, Deg-CPR-1) or with adjuvant only (control). The feeding assays were performed in three replicates and the mean proportions of surviving mites at 24, 48, 72 and 96 h post-feeding, with corresponding 95% confidence intervals (shaded) calculated using Cox’s proportional hazard model with frailty function, are plotted.