Table 4.
Identification of Dermanyssus gallinae proteins present in IgY-immunoreactive spots on a two-dimensional gel immunoblot of the soluble mite extract fractionated by ion exchange chromatography (IEX group 1). Those contigs selected for evaluation as vaccine candidates are given in bold.
| D. gallinae contig number | Top BLASTx hit description and (species) | NCBI accession of top BLASTx hit | % identity | E value | Bit score |
|---|---|---|---|---|---|
| contig00186 | PREDICTED: uncharacterized protein LOC100902161 (Metaseiulus occidentalis) | XP_003741885.1 | 60.8 | 3.09E−53 | 213.4 |
| contig00610 | PREDICTED: fumarate hydratase, mitochondrial-like (Metaseiulus occidentalis) | XP_003742734.1 | 94.3 | 1.65E−70 | 270.8 |
| contig00877 | D-3-phosphoglycerate dehydrogenase, putative (Ixodes scapularis) | XP_002404432.1 | 69.2 | 2.66E−126 | 458.8 |
| contig01284 | PREDICTED: inositol-3-phosphate synthase 1-A-like (Metaseiulus occidentalis) | XP_003740765.1 | 81.5 | 5.43E−42 | 148.3 |
| contig01514 | PREDICTED: serpin B9-like (Metaseiulus occidentalis) | XP_003744662.1 | 36.4 | 0.00025 | 41.2 |
| contig01816 | PREDICTED: aconitate hydratase, mitochondrial-like (Metaseiulus occidentalis) | XP_003744180.1 | 94.0 | 0 | 544.7 |
| contig01795 | PREDICTED: uncharacterized protein LOC100902161 (Metaseiulus occidentalis) | XP_003741885.1 | 56.1 | 2.93E−113 | 415.2 |
| contig02564 | PREDICTED: serpin B9-like (Metaseiulus occidentalis) | XP_003737604.1 | 48.3 | 0 | 544.7 |
| contig02962 | Bifunctional purine biosynthesis protein (Aedes aegypti) | XP_001662952.1 | 75.7 | 1.72E−61 | 142.5 |
| contig03245 | PREDICTED: putative phospholipase B-like 2-like (Metaseiulus occidentalis) | XP_003746012.1 | 57.4 | 0 | 276.9 |
| contig04330 | PREDICTED: inositol-3-phosphate synthase 1-A-like (Metaseiulus occidentalis) | XP_003740765.1 | 78.7 | 4.51E−178 | 631.7 |
| contig05061 | PREDICTED: delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial-like (Metaseiulus occidentalis) | XP_003745491.1 | 65.3 | 1.30E−35 | 154.8 |
| contig07527 | PREDICTED: fumarate hydratase, mitochondrial-like (Metaseiulus occidentalis) | XP_003742734.1 | 80.5 | 1.53E−39 | 167.9 |
| contig11549 | PREDICTED: uncharacterized protein LOC100908877 (Metaseiulus occidentalis) | XP_003744327.1 | 34.3 | 2.33E−08 | 64.3 |
| contig12646 | PREDICTED: aconitate hydratase, mitochondrial-like (Metaseiulus occidentalis) | XP_003744180.1 | 86.2 | 5.00E−66 | 256.1 |