Table 5.
Identification of Dermanyssus gallinae proteins present in IgY-immunoreactive spots on a two-dimensional gel immunoblot of the soluble mite extract fractionated by ion exchange chromatography (IEX group 4). Those contigs selected for evaluation as vaccine candidates are given in bold.
| D. gallinae contig number | 2-DGE immuno-blot | Immuno-affinity enrichment | Top BLASTx hit description and (species) | NCBI accession of top BLASTx hit | % identity | E value | Bit score |
|---|---|---|---|---|---|---|---|
| contig00171 | √ | √ | Vitellogenin 2 (Neoseiulus cucumeris) | AGQ56699.1 | 55.3 | 0 | 751.5 |
| contig00200 | √ | Heat shock protein, putative (Ixodes scapularis) | XP_002407132.1 | 82.0 | 0 | 990.7 | |
| contig00293 | √ | PREDICTED: lysosomal aspartic protease-like (Metaseiulus occidentalis) | XP_003738999.1 | 68.4 | 7.29E−154 | 550.8 | |
| contig00394 | √ | √ | PREDICTED: uncharacterized protein LOC100906494 (Metaseiulus occidentalis) | XP_003742265.1 | 52.8 | 0 | 2631.6 |
| contig00411 | √ | PREDICTED: elongation factor 2-like (Metaseiulus occidentalis) | XP_003744110.1 | 91.7 | 0 | 1558.5 | |
| contig00472 | √ | Heat shock protein 60 (Neoseiulus cucumeris) | AGQ50608.1 | 89.6 | 0 | 989.2 | |
| contig01288 | √ | PREDICTED: eukaryotic initiation factor 4A-II-like (Metaseiulus occidentalis) | XP_003740341.1 | 86.5 | 0 | 657.9 | |
| contig01715 | √ | PREDICTED: alpha-aminoadipic semialdehyde synthase, mitochondrial-like (Metaseiulus occidentalis) | XP_003743993.1 | 87.8 | 3.23E−132 | 477.2 | |
| contig02408 | √ | PREDICTED: V-type proton ATPase subunit B-like (Metaseiulus occidentalis) | XP_003739895.1 | 96.1 | 2.27E−172 | 395.2 | |
| contig03001 | √ | PREDICTED: uncharacterized protein LOC100906494 (Metaseiulus occidentalis) | XP_003742265.1 | 52.4 | 1.85E−80 | 305.8 | |
| contig03511 | √ | PREDICTED: GTP-binding nuclear protein Ran-like (Metaseiulus occidentalis) | XP_003738953.1 | 86.4 | 6.05E−81 | 305.4 | |
| contig04115 | √ | PREDICTED: 78 kDa glucose-regulated protein-like isoform 2 (Metaseiulus occidentalis) | XP_003743478.1 | 79.9 | 2.17E−54 | 219.2 | |
| contig04550 | √ | PREDICTED: alpha-aminoadipic semialdehyde synthase, mitochondrial-like (Metaseiulus occidentalis) | XP_003743993.1 | 85.7 | 9.61E−31 | 86.7 | |
| contig04596 | √ | PREDICTED: glutamate dehydrogenase, mitochondrial-like (Metaseiulus occidentalis) | XP_003737714.1 | 96.9 | 5.61E−98 | 209.1 | |
| contig08052 | √ | PREDICTED: isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial-like (Metaseiulus occidentalis) | XP_003738563.1 | 90.0 | 3.71E−30 | 136.7 | |
| contig08304 | √ | PREDICTED: alpha-aminoadipic semialdehyde synthase, mitochondrial-like (Metaseiulus occidentalis) | XP_003743993.1 | 80.8 | 3.44E−57 | 128.3 | |
| contig08760 | √ | PREDICTED: glutathione reductase-like (Metaseiulus occidentalis) | XP_003748614.1 | 71.0 | 2.60E−15 | 87.4 | |
| contig11091 | √ | PREDICTED: 78 kDa glucose-regulated protein-like isoform 2 (Metaseiulus occidentalis) | XP_003743478.1 | 94.9 | 3.55E−153 | 373.6 | |
| contig11504 | √ | Probable ATP-dependent RNA helicase DDX6 (Strongyloides ratti) | CEF64374.1 | 63.5 | 8.94E−153 | 492.7 | |
| contig11553 | √ | MULTISPECIES: ABC transporter substrate-binding protein (Brucella) | WP_008936495.1 | 32.4 | 0.0448883 | 45.1 | |
| contig11846 | √ | √ | PREDICTED: uncharacterized protein LOC100898154 (Metaseiulus occidentalis) | XP_003738142.1 | 47.3 | 6.04E−64 | 250.8 |
| contig11864 | √ | PREDICTED: glycine N-methyltransferase-like (Metaseiulus occidentalis) | XP_003739110.1 | 77.0 | 1.00E−166 | 481.0 | |
| contig11897 | √ | √ | Vitellogenin 2 (Neoseiulus cucumeris) | AGQ56699.1 | 61.5 | 0 | 1466.8 |
| contig12013 | √ | √ | Vitellogenin 1 (Varroa destructor) | AFN88463.1 | 60.5 | 0 | 2327.4 |
| contig12143 | √ | PREDICTED: heat shock 70 kDa protein cognate 4-like (Metaseiulus occidentalis) | XP_003746930.1 | 78.5 | 0 | 664.8 | |
| contig12579 | √ | PREDICTED: uncharacterized protein LOC100898154 (Metaseiulus occidentalis) | XP_003738142.1 | 43.3 | 8.65E−57 | 226.5 | |
| contig13080 | √ | PREDICTED: actin-5C-like (Metaseiulus occidentalis) | XP_003742602.1 | 98.1 | 0 | 748.0 | |
| contig13089 | √ | √ | PREDICTED: uncharacterized protein LOC100898154 (Metaseiulus occidentalis) | XP_003738142.1 | 43.9 | 9.90E−65 | 253.4 |
| contig13090 | √ | PREDICTED: actin, cytoplasmic 2-like isoform 2 (Metaseiulus occidentalis) | XP_003736979.1 | 99.1 | 1.16E−55 | 221.5 | |
| contig13094 | √ | PREDICTED: uncharacterized protein LOC100900885 (Metaseiulus occidentalis) | XP_003745143.1 | 73.2 | 0 | 764.2 | |
| contig13207 | √ | √ | Large lipid transfer protein (Varroa destructor) | AGW50714.1 | 42.7 | 0 | 356.7 |
| contig13301 | √ | Actin 3 (Neoseiulus cucumeris) | AGQ50606.1 | 97.8 | 7.63E−76 | 288.5 | |
| contig13306 | √ | PREDICTED: glutamine synthetase 1, mitochondrial-like (Metaseiulus occidentalis) | XP_003747444.1 | 84.0 | 0 | 339.7 | |
| contig13317 | √ | √ | Vitellogenin 2 (Neoseiulus cucumeris) | AGQ56699.1 | 60.0 | 0 | 1870.5 |
| contig13348 | √ | PREDICTED: ATP synthase subunit beta, mitochondrial-like isoform 1 (Metaseiulus occidentalis) | XP_003745616.1 | 83.6 | 0 | 831.2 |
2-DGE, two-dimensional gel electrophoresis.