Figure 2.

Tissue morphology of the kidney cortex in control (ctrl) and ethylene glycol (EG)-treated (eg) female and male rats in tissue cryosections immunostained with actin-Ab. In the kidneys of control animals of both sexes, we observed regular dense arrangements of undamaged glomeruli (G), closed proximal convoluted tubules with thick epithelium and brightly-stained brush-border (arrowheads), and distal tubules and collecting ducts with narrow or closed lumen (arrows). In the kidney cortex of the EG-treated animals of both sexes, distal tubules and collecting ducts had thin epithelium and distended lumen (arrows). In the EG-treated males, many proximal convoluted tubules had wide lumen, thin epithelium, and variable loss of brush border (arrowheads). Images represent findings in 10 animals from each experimental group. Bar, 50 μm.