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. 2015 Nov 18;10:7109–7128. doi: 10.2147/IJN.S94045

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© 2015 Gao et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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The effect of functionalized nanofibers on osteogenic differentiation of hMSCs under non-osteoinductive condition.

Notes: (A) Representative staining of ALP on day 14 and determination of ALP activity at 7 and 14 days. (B) ARS staining and determination of calcium deposition on day 21. (C) RT-qPCR analysis for osteo-specific genes on day 14. (D) Representative immunofluorescent images of OCN (green) and OPN (red) in different groups on day 14. Scale bars indicate 100 μm. (E) Western blot analysis of Col1a1 and Runx2 expression in hMSCs on different samples. ^#Compared with R-pDA, P<0.05; *compared with A-pDA, P<0.05; ^$compared with R-pDA-pep, P<0.05.

Abbreviations: hMSCs, human mesenchymal stem cells; ALP, alkaline phosphatase; ARS, Alizarin Red S; RT-qPCR, real-time quantitative polymerase chain reaction; OCN, osteocalcin; OPN, osteopontin; Col1a1, type I collagen alpha 1; Runx2, Runt-related transcription factor 2; pDA, polydopamine; TCP, tissue culture plate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PCL, polycaprolactone; R, pure randomly oriented PCL nanofiber; A, pure aligned PCL nanofiber; pep, peptide.