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. 2015 Nov 18;10:7129–7143. doi: 10.2147/IJN.S92179

Figure 3.

Figure 3

Gel electrophoresis of HSV-TK amplified by RT-PCR after pHsp 70-HSV-TK transfected into SMMC-7721 cells.

Notes: Lane 1, marker (each band in turn from bottom to top is 100, 300, 500, 750, 1,000, and 2,000 bp); lane 2, infected cells that did not receive hyperthermia with only one strip, 110 bp of GAPDH; lane 3, untransfected SMMC-7721 cells with only one strip, 110 bp of GAPDH; lane 4, infected cells that receive hyperthermia with two clear strips, 496 bp of HSV-TK and 110 bp of GAPDH used as reference gene.

Abbreviations: bp, base pair; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HSV-TK, herpes simplex virus thymidine kinase; RT-PCR, reverse transcription-polymerase chain reaction.