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. 2015 Nov 18;10:7129–7143. doi: 10.2147/IJN.S92179

Figure 7.

Figure 7

Gel electrophoresis of HSV-TK amplified by RT-PCR after pHsp 70-HSV-TK transfected into SMMC-7721 cells.

Notes: Lane 1, the thermotherapy-alone group with only one strip, 110 bp of GAPDH; lane 2, the gene transfection-alone group with only one strip, 110 bp of GAPDH; lane 3, the blank control group with only one strip, 110 bp of GAPDH; lanes 4–6, gene therapy combined with heating therapy group with two clear strips, 496 bp of HSV-TK and 110 bp of GAPDH used as reference gene; lane 7, marker (each band in turn from bottom to top is 100, 300, 500, 750, 1,000, and 2,000 bp).

Abbreviations: bp, base pair; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HSV-TK, herpes simplex virus thymidine kinase; RT-PCR, reverse transcription-polymerase chain reaction.