Fig. 5.

FFAR4 was expressed in cardiac myocytes and nonmyocytes (fibroblasts). A: FFAR expression in mouse heart. RNA was isolated from adult mouse heart and mRNA levels of FFAR1–4 were measured by semi-quantitative RT-PCR (n = 4). B: FFAR4 expression in whole heart (H), cardiac myocytes (M), and nonmyocytes (fibroblasts) (N). RNA was isolated from freshly isolated adult mouse cardiac myocytes and nonmyocytes (fibroblasts) and mRNA levels of FFAR4 were measured by qualitative PCR. PCR products for FFAR4 (520 bp) and GAPDH (326 bp) are indicated.