Fig. 2.

Deficiency in myeloid cell ABCG1 modulates ATM cholesterol levels. WT male C57BL/6 mice were irradiated and then two separate groups were engrafted with bone marrow from either WT (WT-BMT) (open bars) or Abcg1^−/− (Abcg1^−/− BMT) mice (black bars) (A–C). Animals were fed an HFD for 13 weeks and then subjected to 3 weeks of caloric restriction or maintained ad libitum on the HFD. ATMs were collected using FACS as described in Materials and Methods. Total (A), unesterified (B), and esterified (C) cholesterol levels were quantified using LC/MS/MS. Values are presented as mean ± SEM (n = 5), and P values are shown in the figure.