Fig. 3.

mRNA levels for cholesterol metabolism and inflammatory genes in epididymal tissues taken from mice. WT-BMT (open bars) and Abcg1^−/− BMT (black bars) were treated as described in Figure 2. mRNA was isolated and PCR performed as described in Materials and Methods. mRNA results are expressed as fold induction as compared with WT-BMT (WT-BMT = 1 arbitrary unit). Values are presented for ACAT (A), MCP1 (B), and IL-6 (C). Data are expressed as mean ± SEM for n = 8 mice. ** P < 0.03 between WT-BMT and Abcg1^−/− BMT. # P < 0.003 between ad libitum and caloric restriction treatments within the same mouse strain.