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Isothermal titration calorimetry measurement to determine the interaction of Psl and salmon sperm DNA.
A. Salmon sperm DNA to titrate Psl dispersion.
B. The genomic DNA of P. aeruginosa to titrate Psl dispersion. The upper panel shows raw titration data, and the bottom panel is integrated and dilution-corrected peak area plotting of titration data. To a Psl dispersion (2.25 mg ml−1), DNA (200 μg ml−1) is titrated in 10 μl portion.
