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Loss of supercoiled pGEM-3Zf (–) DNA versus electron fluence. After irradiation, DNA was untreated (closed circles) or incubated at 37 °C (closed triangles) or incubated with Nth (asterisk) or Fpg (dashes) or both enzymes Nth and Fpg (pluses). Linear fits obtained from data recorded at electron fluences < 1012 electrons/cm2.
