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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Biochemistry. 2015 Oct 30;54(44):6704–6711. doi: 10.1021/acs.biochem.5b00567

Figure 2.

Figure 2

Pramlintide (PM) is a more effective inhibitor of amyloid formation by wild type hIAPP than is rIAPP. (A) The kinetics of hIAPP amyloid formation in the presence of rIAPP monitored by thioflavin-T fluorescence assays. Black, hIAPP; red, a 1:1 mixture of hIAPP and rIAPP; green, a mixture of hIAPP and rIAPP at a 1 to 2 ratio; blue, a mixture of hIAPP and rIAPP at a 1 to 5 ratio; pink, a mixture of hIAPP and rIAPP at a 1 to 10 ratio; grey, 160 μM rIAPP. (B) The kinetics of hIAPP amyloid formation in the presence of PM monitored by thioflavin-T fluorescence essays. The same color coding is used as in panel A. Experiments were conducted in 20 mM Tris-HCl (pH 7.4), without stirring at 25 °C. The concentration of hIAPP was 16 μM.