Figure 1. FL-Myhc_334-352 induces both CD4 and CD8 T cell responses.

A/J mice were immunized with FL-Myhc_334-352 in CFA; after 14 days animals were euthanized to prepare single cell suspensions from spleens and lymph nodes, from which CD4 and CD8 T cells were enriched by magnetic separation. The purity of CD4 and CD8 T cell preparations were ascertained by staining with antibodies for CD3, CD4 and CD8, and 7-AAD, where the percentages of respective cell populations were determined within the live (7-AAD⁻) cells (top panel). Cells were stimulated with the irradiated APCs pulsed with the indicated peptides for two days. After pulsing with ³[H]-thymidine (1μCi/well) for 16 hours, proliferative responses were measured as cpm. Mean ± SEM values derived from five experiments, each involving 5 to 8 mice are shown (bottom panels). RNase_43-56 (control).