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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Int J Cardiol. 2015 Sep 18;202:311–321. doi: 10.1016/j.ijcard.2015.09.016

Figure 3. Evaluation of CD4 and CD8 T cell responses to the truncated peptides of FL-Myhc334-352 (13 to 16-mers) in the primary screening.

Figure 3

A/J mice were immunized with Myhc334-352 in CFA; after 14 days, single cell suspensions were prepared from spleens and lymph nodes, from which CD4 and CD8 T cells were enriched by magnetic separation. Cells were stimulated with Myhc334-352 and its truncated peptides (13 to 16-mers: Trunk A to D) in the presence of irradiated APCs for two days, followed by pulsing with 3[H]-thymidine. After 16 hours, proliferative responses were measured as cpm. RNase43-56 (control). Mean ± SEM values obtained from two individual experiments, each involving 10 to 11 mice are shown.