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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Int J Cardiol. 2015 Sep 18;202:311–321. doi: 10.1016/j.ijcard.2015.09.016

Figure 4. Evaluation of CD4 and CD8 T cell responses to the short peptides of FL-Myhc334-352 (10 to 11-mers) in the secondary screening.

Figure 4

A/J mice were immunized with Myhc334-352 in CFA; after 14 days, CD4 and CD8 T cells were enriched by magnetic separation using the cell suspensions obtained from spleens and lymph nodes. Cells were stimulated with Myhc334-352 and short peptides (10 to 11-mers: Trunk A-4 to A-6) in the presence of irradiated APCs for two days, followed 16 hours later by pulsing with 3[H]-thymidine, the incorporation of which was measured as cpm. RNase43-56 (control). Mean ± SEM values obtained from two to four individual experiments, each involving 9 to 11 mice are shown.