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. Author manuscript; available in PMC: 2016 Nov 19.
Published in final edited form as: Mol Cell. 2015 Nov 5;60(4):685–696. doi: 10.1016/j.molcel.2015.10.009

Figure 5.

Figure 5

Assessment of mitophagy using mt-Keima and Stimulated Emission Depletion (STED) microscopy. A) Representative STED image of mt-Keima MEFs showing merged red and green fluorescence. B) The red only mt-Keima signal. C) Merged red mt-Keima signal with LysoSensor fluorescence (depicted in Cyan; scale bar=2 μm). D) Higher magnification of the mt-Keima red signal within lysosomes. Arrows indicate what appears to be a recently ingested autophagosome decorated with mt-Keima and contained within a presumed newly formed autolysosome (scale bar=1 μm).