Skip to main content
. Author manuscript; available in PMC: 2016 Nov 20.
Published in final edited form as: Science. 2015 Nov 20;350(6263):932–937. doi: 10.1126/science.aad0814

Fig. 1.

Fig. 1

Generation of Spo11-oligo maps. (A) Meiotic recombination. Spo11 generates covalent protein-linked DSBs; endonucleolytic cleavage (grey arrowheads) liberates Spo11 bound to short oligos. DSB ends are resected and repaired to yield crossover or non-crossover products. The broken chromosome (black) copies information from the uncut allele (teal). (B) Schematic of Saccharomyces phylogeny (18, 20). Black, species/strains in this study; genic sequence divergence from S. cerevisiae (42) and estimated time since last common ancestor (20) are shown. YPS, YPS128; UW, UWOPS03-461.4. (C,D) Conserved sizes of Spo11 oligos. Immunoprecipitated, radiolabelled Spo11-oligo complexes were separated by SDS-PAGE (C), or were digested with proteinase K and resolved on a denaturing polyacrylamide gel (D). Autoradiographs (with lane traces in D) are shown. (E) Reproducibility of S. kudriavzevii Spo11-oligo maps. RPM, reads per million mapped; profiles were smoothed with 201-bp Hann window.