Fig. 2.

Effect of WRPH-pa in different hydrolytic conditions on MCF-7 cell proliferation. a WRPH-pa was hydrolyzed at temperature of 50, 55, 60, 65 and 70 °C, respectively with the conditions of time 3 h, substrate concentration 4 % and an enzyme-substrate ratio 10 %. b WRPH-pa was hydrolyzed at time of 1, 2, 3, 4 and 5 h, respectively with the conditions of temperature 60 °C, substrate concentration 4 % and an enzyme-substrate ratio 10 %. c WRPH-pa was hydrolyzed at substrate concentrations of 2, 3, 4, 5 and 6 %, respectively with the conditions of temperature 60 °C, time 3 h and an enzyme-substrate ratio 10 %. d WRPH-pa was hydrolyzed at enzyme-substrate ratio of 8, 9,10,11 and 12 %, respectively with the conditions of temperature 60 °C, time 3 h and substrate concentration 4 %. WRPH-pa, walnut residual protein hydrolysate prepared with papain