Fig. 5.

Induction of apoptosis cell death by CTLEW in MCF-7 cells. a Annexin V-FITC/PI doubles staning assay. Cells were treated with (0.5 or 1.0 mg/ml) or without (control) CTLEW for 48 h, and were stained by annexinV-FITC/PI, respectively. Phosphotidylserine externalization in MCF-7 cells was determined by flow cytometry of annexinV-FITC/PI stained cells. b TUNEL apoptosis assay. Cells were treated with (0.5 or 1.0 mg/ml) or without (control) CTLEW for 48 h, cell tissue slice was labelled by digoxigenin and observed by immunocytochemical staining, the labelled yellow brown nucleus represents the apoptosis cells. Data were presented as mean ± S.D. of three separate experiments