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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cell Microbiol. 2015 Jan 26;17(6):876–892. doi: 10.1111/cmi.12409

Figure 5. Effects of brefeldin A (BFA) on protein secretion and Listeria protrusion formation.

Figure 5

Caco-2 BBE1 cells were transfected with a plasmid expressing Secreted Placental Alkaline Phosphatase (SEAP). Approximately 72 h later, cells were used for analysis of SEAP secretion or bacterial protrusion formation. A. SEAP activity. After treatment of Caco-2 BBE1 cells with the indicated concentrations of BFA (+) or with the vehicle DMSO for 1 h, levels of secreted (extracellular) SEAP were measured as described in the Experimental Procedures. *, P < 0.05 relative to cells treated with DMSO (−). B. Listeria protrusion formation. Caco-2 BBE1 cells were infected with wt or ΔinlC bacterial strains for 1 h followed by incubation for another 4.5 h in medium containing 5 μg/ml BFA (+) or DMSO (−). Samples of fixed and labeled cells were analyzed by confocal microscopy for quantification of bacterial protrusions. *, P < 0.05 relative to DMSO-treated cells (−) infected with wt Listeria. Data in A and B are mean +/− SEM from three experiments.