Table 1.

Endothelial tube formation in TLR2KD and TLR4KD cells.

Time (min)	Nonspecific siRNA	TLR2 siRNA	TLR4 siRNA
120	2.88 ± 0.93	1.75 ± 0.25	1.50 ± 0.71
150	12.00 ± 1.96	10.50 ± 1.15	9.88 ± 1.89
180	15.75 ± 1.85	18.00 ± 1.12	17.00 ± 2.73
210	20.13 ± 2.08	20.40 ± 1.74	22.10 ± 2.37
240	21.38 ± 1.93	21.90 ± 1.26	25.00 ± 2.76
270	22.88 ± 1.59	21.80 ± 1.70	25.60 ± 2.68
300	23.75 ± 1.81	19.90 ± 1.80	25.90 ± 2.13a
330	20.25 ± 2.31	17.00 ± 1.64	23.00 ± 1.79b
360	14.60 ± 2.63	11.60 ± 0.68	18.20 ± 2.24b

HDMVECs were placed in the wells of angioslides coated with growth factor–reduced Matrigel after transfection with siRNA to a nonspecific target, TLR2 or TLR4. After allowing the cells to settle for 2 h, tube formation was imaged every 15 min for a total of 360 min. The number of tubes visible in each frame was calculated at 30-min intervals for each treatment group.

^ap < 0.05;

^bp < 0.03 TLR4KD cells versus TLR2KD cells. N = 3 experiments.